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Equipment:
Featured Product
Thermo Scientific* Pulsed-Q
Dissociation (PQD) is a patented fragmentation technique that
eliminates the low mass cut-off for Thermo Scientific linear ion trap
mass spectrometers and allows quantitation with isobaric peptide tags.
PQD is a novel
fragmentation mechanism developed specifically for Thermo Scientific LTQ
series mass spectrometers.?t involves precursor ion activation at high
Q, a time delay to allow the precursor to fragment, then a rapid pulse
to low Q where all fragmentions are trapped. The product ions can then
be scanned out of the ion trap and detected. PQD fragmentation produces
precise, reproducible fragmentation and has been used for peptide
quantitation on the LTQ series using both electrospray and MALDI source
ionization. PQD fragmentation of peptides produces robust precursor ion
fragmentation, and provides the ability to routinely scan product ions
down to m/z 50. Fragmentation occurs in about the same time scale as
Collision Induced Dissociation (CID) and can easily be incorporated into
general Xcalibur methods and used in conjunction with CID, MSn,
and other scan functions.
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Equipment
In combination with the High Sensitivity Protein 250 kit, the Agilent 2100 Bioanalyzer detects proteins down to 1 pg/μL on chip, which is superior to silver stain SDS-PAGE. It provides quantification over a dynamic range of up to 4 orders of magnitude, is highly reproducible, which is particularly important for the analysis and quality control of proteins. Traditionally, silver stain methods were used in electrophoresis for protein detection at the low picogram range.
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 Bruker Daltonics provides a variety of innovative mass spectrometry systems. Our powerful, yet easy to implement, products are specifically designed to meet the rapidly growing needs of customers in the academic, pharmaceutical, industrial, clinical, and defense areas. Consistently and expertly supported, our turnkey system solutions and complete workflows offer integrated instrument and software tools which enhance the productivity and capabilities of any analytical operation. Bruker Daltonics product lines include platforms representing many types of separation technologies and mass spectrometry including:
Our systems are also capable of integration with a number of separation techniques including HPLC, Fast Chromatography, Nano-LC, Gas Chromatography (GC) and even Capillary Electrophoresis (CE). |
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 The highest performing LC/MS/MS tools to accelerate your lab. As the leader in mass spectrometry for nearly thirty years, AB SCIEX has never stopped striving to create the best products available, period. We're continuing that tradition of leadership with our new, built-from-the-ground-up mass spectrometry platform for both our triple quadrupole and QTRAP® high-performance systems. Scientists worldwide trust us to provide optimum productivity in quantitative and qualitative applications ranging from pharmaceutical and biomarker analysis, to food, forensics, clinical research, and environmental analysis.
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